Tobey Maguire
Peter Parker/Spider-Man
Kirsten Dunst
Mary Jane Watson
James Franco
Harry Osborn
Willem Dafoe
Norman Osborn/Green Goblin
Cliff Robertson
Wujek Ben Parker
Rosemary Harris
Ciocia May Parker
J.K. Simmons
J. Jonah Jameson
Joe Manganiello
Flash Thompson
Bill Nunn
Joseph „Robbie” Robertson
Elizabeth Banks
Betty Brant
Ted Raimi
Hoffman
Ron Perkins
Dr. Mendell Stromm
Gerry Becker
Maximilian Fargas
Jack Betts
Henry Balkan
Stanley Anderson
Generał Slocum
K.K. Dodds
Ellie Simkins
Michael Papajohn
Złodziej samochodu (Dennis Carradine)
Randy Mario Poffo
Bone Saw McGraw
Bruce Campbell
Spiker ringowy
