Tobey Maguire
Peter Parker/Spider-ManKirsten Dunst
Mary Jane WatsonJames Franco
Harry OsbornWillem Dafoe
Norman Osborn/Green GoblinCliff Robertson
Wujek Ben ParkerRosemary Harris
Ciocia May ParkerJ.K. Simmons
J. Jonah JamesonJoe Manganiello
Flash ThompsonBill Nunn
Joseph „Robbie” RobertsonElizabeth Banks
Betty BrantTed Raimi
HoffmanRon Perkins
Dr. Mendell StrommGerry Becker
Maximilian FargasJack Betts
Henry BalkanStanley Anderson
Generał SlocumK.K. Dodds
Ellie SimkinsMichael Papajohn
Złodziej samochodu (Dennis Carradine)Randy Mario Poffo
Bone Saw McGrawBruce Campbell
Spiker ringowy