- Tobey Maguire
Peter Parker/Spider-Man - Kirsten Dunst
Mary Jane Watson - James Franco
Harry Osborn - Alfred Molina
Dr. Otto Octavius/Dr. Octopus - Rosemary Harris
Ciocia May Parker - J.K. Simmons
J. Jonah Jameson - Donna Murphy
Rosalie Octavius - Daniel Gillies
John Jameson - Bill Nunn
Joseph „Robbie” Robertson - Elizabeth Banks
Betty Brant - Ted Raimi
Hoffman - Dylan Baker
Dr. Curt Connors - Cliff Robertson
Wujek Ben Parker - Willem Dafoe
Norman Osborn - Elya Baskin
Mr. Ditkovich - Mageina Tovah
Ursula - Joel McHale
Mr. Jacks - Bruce Campbell
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