- Shameik Moore
Miles Morales/Spider-Man - Jake Johnson
Peter B. Parker/Spider-Man - Hailee Steinfeld
Gwen Stacy/Spider-Woman - John Mulaney
Peter Porker/Spider-Ham - Kimiko Glenn
Peni Parker/SP//dr - Nicolas Cage
Peter Parker/Spider-Man Noir - Chris Pine
Peter Parker/Spider-Man - Zoë Kravitz
Mary Jane Watson-Parker - Lily Tomlin
May Parker - Brian Tyree Henry
Jefferson Davis - Luna Lauren Velez
Rio Morales - Mahershala Ali
Aaron Davis/Prowler - Liev Schreiber
Wilson Fisk/Kingpin - Lake Bell
Vanessa Fisk - Kathryn Hahn
Olivia Octavius/Doctor Octopus - Marvin „Krondon” Jones III
Lonnie Lincoln/Tombstone - Joaquín Cosío
Scorpion - Jorma Taccone
Norman Osborn/Green Goblin