- Robbie Daymond
Peter Parker / Spider-Man
- Nancy Linari
Aunt May Parker
- Patton Oswalt
Uncle Ben Parker
- Max Mittelman
Harry Osborn
- Laura Bailey
Gwen Stacy
- Nadji Jeter
Miles Morales
- Melanie Minichino
Anya Corazon
- Fred Tatasciore
Max Modell
- John DiMaggio
Raymond Warren / Jackal
- Ben Diskin
Spencer Smythe
- Jason Spisak
Alistair Smythe
- Zeno Robinson
Randy Robertson
- Alastair Duncan
Adrian Toomes / Vulture
- Josh Keaton
Norman Osborn
- Scott Menville
Otto Octavius / Doctor Octopus
- Cameron Boyce
Herman Schultz / Shocker
- Natalie Lander
Liz Allan
- Matthew Mercer
Aleksei Sytsevich / The Rhino
- Joe Quesada
Joe
- Steven Blum
Bonesaw McGee
